Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Strain, strain background (Mus musculus) | Slc17a7-/-(VGLUT1 KO) mice | PMID: 15103023 | available upon request to Dr Sonja M. Wojcik wojcik@em.mpg.de | |
| Strain, strain background (Mus musculus) | Slc17a7v/v(VGLUT1venus KI) mice | PMID: 22031900 | available upon request to corresponding author | |
| Transfected construct (Homo sapiens) | F(syn)W-RBN::Synaptobervin2-EGFP | PMID: 23581566 | available upon request to Dr. Etienne Herzog |
Figure 1, Figure 3 and related results part. Lentiviral vector expressing Syb2EGPF. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1-venus | PMID: 23581566 | available upon request to Dr. Etienne Herzog |
Figure 1, Figure 4 and related results part. Lentiviral vector expressing VGLUT1venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT2-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 1—figure supplement 1–S1 and related results part. Lentiviral vector expressing VGLUT2venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 ∆C-term-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1∆C-term-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 DQL514AQA-venus |
This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1DQL514AQA-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 ∆PRD1+2-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1∆PRD1+2-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 PP534AA-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1PP534AA-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 S540A-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1S540A-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 T544A-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1T544A-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 ∆PRD2-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1∆PRD2-venus. |
| Transfected construct (Rattus norvegicus) | F(syn)W-RBN::VGLUT1 P554A-venus | This paper | available upon request to Dr. Etienne Herzog |
Figure 4 and related results part. Lentiviral vector expressing VGLUT1P554A-venus. |
| Transfected construct (Rattus norvegicus) | AAV9::VGLUT1mCherry-miniSOG | This paper | available upon request to Dr. Etienne Herzog |
Figure 3 and related results part. Lentiviral vector expressing VGLUT1mCherry-miniSOG. |
| Transfected construct (Rattus norvegicus) | AAV9::sVGLUT1mCherry-miniSOG | This paper | available upon request to Dr. Etienne Herzog |
Figure 3 and related results part. Lentiviral vector expressing sVGLUT1mCherry-miniSOG. |
| Transfected construct (Homo sapiens) | AAV9::mCerulean3-EndophilinA1SH3 | This paper | available upon request to Dr. Etienne Herzog |
Figure 5 and related results part. Lentiviral vector expressing mCerulean3-EndophilinA1SH3. |
| Transfected construct (Homo sapiens) | AAV9::mCerulean3-EndophilinA1SH3E329K, S336K | This paper | available upon request to Dr. Etienne Herzog |
Figure 5 and related results part. Lentiviral vector expressing mCerulean3-EndophilinA1SH3E329K, S336K. |
| Transfected construct (Homo sapiens) | AAV9::mCerulean3-IntersectinSH3B | This paper | available upon request to Dr. Etienne Herzog |
Figure 5 and related results part. Lentiviral vector expressing mCerulean3-IntersectinSH3B. |
| Antibody | GFP, Mouse, monoclonal | Roche | Cat. 11814460001 RRID:AB_390913 |
1:1000 |
| Antibody | VIAAT, Guinea pig, polyclonal | SYSY | Cat. 131004 RRID:AB_887873 |
1:1000 |
| Antibody | VGLUT2, Guinea pig, polyclonal | Millipore | Cat. AB2251 RRID:AB_1587626 |
1:2000 |
| Antibody | VGLUT1, guinea pig, polyclonal | Merck | Cat. AB5905 RRID:AB_2301751 |
1:5000 |
| Antibody | EndophilinA1, rabbit, polyclonal | PMID: 16606361 | available upon request to Dr. Etienne Herzog | 1:500 |
| Peptide, recombinant protein | FastAP Thermosensitive Alkaline Phosphatase | Thermo Scientific | Cat. EF0651 | |
| Peptide, recombinant protein | Haltphosphatase inhibitor cocktail | Thermo Scientific | Cat. 78420 | |
| Chemical compound, drug | Phos-tag Acrylamid | Wako | Cat. AAL-107 | |
| Software, algorithm | FRAP Analysis Plugin | This paper | The plugin is available at: https://github.com/fabricecordelieres/IJ-Macro_FRAP-MM | |
| Software, algorithm | KymoToolbox Plugin | PMID: 23374344 | The plugin is available at: https://github.com/fabricecordelieres/IJ-Plugin_KymoToolBox |