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. 2019 Nov 2;7(1):e000751. doi: 10.1136/bmjdrc-2019-000751

Figure 1.

Figure 1

Diet-induced obesity (DIO) and type 2 diabetes (T2D) characteristics were reversed by subsequent weight loss. (A) Male C57BL/6 mice were fed a LFD (n=24) or HFD (n=42) diet for 10 weeks when 12 LFD and 14 HFD mice were sacrificed. Fourteen of the remaining HFD mice were shifted to a LFD for 10 weeks. The rest of the mice continued with their original diet. (B) Mouse bodyweight was measured once a week. (C) Blood glucose levels were measured over time after 5 hours of fasting and subsequent intraperitoneal injections of glucose. Glucose area under the curve (AUC) was calculated per group. (D) Blood glucose levels were measured over time after 4 hours of fasting and subsequent intraperitoneal injections of insulin. Glucose AUC was calculated per group. (E) Fasting plasma triglycerides and cholesterol levels. (F) Plasma cytokines (TNF, CXCL1, and IL-10) were measured using an electrochemiluminescence assay. (G) H&E stained visceral fat sections. Scale bar length: 100 µm. (H) H&E stained fat sections were scored for the count of infiltrating immune cells as a measure of inflammation. (I) H&E stained liver sections. Scale bar length: 100 µm. (J) H&E stained liver sections were scored for the count of infiltrating immune cells as a measure of inflammation. Error bars represent SD. AUC, area under the curve; CXCL1, C-X-C motif chemokine ligand 1; GTT, glucose tolerance test; HFD, high-fat diet; IL-10, interleukin-10; ITT, insulin tolerance test; LFD, low-fat diet; TNF, tumor necrosis factor.