Table 1. Biomolecular artificial hydrolases.
| Amino-acid sequence | Molecular mass (Da) | Substrate | kcat/Km (M−1 s−1) | kcat/Km × 10−2 ((g l−1)−1 s−1) | Refs. |
|---|---|---|---|---|---|
| F | 165 | pNPA | 76.54a and 10.62b (pH 7.0) | ~46a and ~6.4b | This study |
| Carbonic anhydrase (CA II) | ~29,000 | pNPA | 1,670 (pH 7.0) | ~5.7 | 46 |
| Ac-IHIHIYI-NH2 | 949 | pNPA | 355 (pH 8.0) | ~37 | 18 |
| Ac-IHIHIQI-NH2 | 914 | pNPA | 62 (pH 8.0) and 15.76 (pH 7.3) | ~6.7 and ~1.7 | 11,13 |
| HKH-LLLAAA(K)-palmitoyl | 1,338 | DNPAc | 19.76 (pH 7.4) | ~1.4 | 12 |
| HSGVKVKVKVKVDPPTKVKVKVKV-NH2 | 2,510 | pNPA | 19.18 (pH 9.0) | ~0.76 | 14 |
| Fmoc-FFH-NH2 + Fmoc-FFR-NH2 | 671 + 689 | pNPA | 1.82 (pH 7.5) | ~0.13 | 16 |
| Ac-GLKALEEKCKALEEKLKALEEKHKALEEKG-NH2 | 3,432 | pNPA | 1.38 (pH 7.5) | ~0.04 | 10 |
| RSGQQKFQFQFEQQ- NH2 + HSGQQKFQFQFEQQ- NH2 | 1,785 + 1,766 | pNPA | 0.15 (pH 7.4) | 4.2×10−5 | 17 |
a
In deionized water.
b
In Tris–HCl (25 mM) buffer solution.
c
2,4-dinitrophenyl acetate (DNPA).