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. 2019 Nov 12;13:507. doi: 10.3389/fncel.2019.00507

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Copyright © 2019 Hua, Yang, Chen, Zhang, Hu and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The protective effects of PNU-282987 on cell viability of primary cultured astrocytes after MPP⁺ injury. A 30-min pretreatment with PNU-282987 (0.001, 0.01, 0.1, 1, 10, or 100 μM) inhibited 800 μM MPP⁺-induced cultured astroglial death after 24 h. (A) Pretreatment with PNU-282987 significantly promoted the viability of astrocytes in a concentration-dependent manner after MPP⁺ injury. (B) Pretreatment with PNU-282987 decreased the release of LDH from astrocytes in a concentration-dependent manner after MPP⁺ injury. (C) Pretreatment with 10 μM PNU-282987 with 100 nM MLA abolished the increased viability of astrocytes shown in the pretreatment with PNU-282987 only after MPP⁺ injury. (D) Pretreatment with 10 μM PNU-282987 with 100 nM MLA abolished the reduced release of LDH from astrocytes shown in the pretreatment with PNU-282987 only after MPP⁺ injury. Data are presented as mean ± SEM of three independent experiments. ^∗∗∗p < 0.001 vs. control group; ^###p < 0.001, ^##p < 0.01 vs. MPP⁺ treatment group; ^$$$ p < 0.001, ^$$ p < 0.01 vs. PNU-282987 treatment group. Con, control; PNU, PNU-282987; MLA, methyllycaconitine; LDH, lactate dehydrogenase.