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. 2019 Nov 12;13:507. doi: 10.3389/fncel.2019.00507

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Copyright © 2019 Hua, Yang, Chen, Zhang, Hu and Fan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The protective effects of PNU-282987 on MPP⁺-induced apoptosis in primary cultured astrocytes. (A) Apoptotic cells characterized by cell shrinkage and chromatin condensation were examined by Hoechst 33342 staining and indicated by a yellow arrow. Scale bar: 200 μm. (B) Quantification for staining analysis was presented. (C) Apoptosis cells were stained with Annexin-V and propidium iodide and analyzed by flow cytometry. Representative picture and quantification for analysis treatment were shown. (D) Quantification for flow cytometry analysis was presented. Data are presented as mean ± SEM of three independent experiments. ^∗∗∗p < 0.001, ^∗∗p < 0.01, and ^∗p < 0.05 vs. control group; ^###p < 0.001 and ^#p < 0.05 vs. MPP⁺ treatment group; ^$$$p < 0.001 and ^$p < 0.05 vs. PNU-282987 treatment group. Con, control; PNU, PNU-282987.