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. 2019 Nov 12;13:507. doi: 10.3389/fncel.2019.00507

FIGURE 4.

FIGURE 4

The effects of PNU-282987 on MPP+-induced expression of JNK-p53-caspase-3 signaling pathway in primary cultured astrocytes. (A) Representative immunoblot of p-JNK, JNK, p-p53, p53, cleaved-caspase-3, caspase-3, Bax, and Bcl-2 in cultured astrocytes. (B) Quantitation of p-JNK and JNK levels at baseline and in response to MPP+ and PNU-282987 with or without MLA. (C) Quantitation of p-p53 and p53 levels at baseline and in response to MPP+ and PNU-282987 with or without MLA. (D) Quantitation of cleaved-caspase-3 and caspase-3 levels at baseline and in response to MPP+ and PNU-282987 with or without MLA. (E) Quantitation of Bax and Bcl-2 levels at baseline and in response to MPP+ and PNU-282987 with or without MLA. Data are presented as mean ± SEM of three independent experiments. ∗∗∗p < 0.001, ∗∗p < 0.01, and ∗p < 0.05 vs. control group; ###p < 0.001, ##p < 0.01, and #p < 0.05 vs. MPP+ treatment group; $$$p < 0.001 and $$p < 0.01 vs. PNU-282987 treatment group. Con, control; PNU, PNU-282987; MLA, methyllycaconitine.