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. 2019 Nov 12;13:507. doi: 10.3389/fncel.2019.00507

FIGURE 5.

FIGURE 5

α7nAChR deficiency abrogates the protective effects of PNU-282987 on primary cultured astrocytes after MPP+ injury. (A) The effects of PNU-282987 on the viability of cells were abolished in cultured astrocytes from KO mice. (B) The effects of PNU-282987 on the release of LDH were abolished in cultured astrocytes from KO mice. (C) Apoptotic cells characterized by cell shrinkage and chromatin condensation were examined by Hoechst 33342 staining and indicated by a yellow arrow. Scale bar: 200 μm. (D) Quantification for Hoechst 33342 staining analysis was presented. Data are presented as mean ± SEM of three independent experiments. ∗∗∗p < 0.001 and ∗∗p < 0.01 vs. corresponding control group; ###p < 0.001 and ##p < 0.01 vs. corresponding MPP+ treatment group; $$p < 0.01 vs. PNU-282987-treated WT group. WT, astrocyte from wild-type mice; KO, astrocytes from α7nAChR KO mice; PNU, PNU-282987; LDH, lactate dehydrogenase.