Figure 5.

Biological activities of PJ compositions in normal hepatocytes and HepG2 cells. (A) Cells viability rate and indicated CC₅₀ of purified PJ glycosides on normal hepatocytes cells that were pre-treated with different concentrations (0–1.5 mg/ml) of each PJ. (B) Cell viability rate and CC₅₀ of PJ products on treated-HepG2 cells using WST-1 assay. Error bars indicate the SD of four different replicates. (C) Fold change in steady-state mRNA of IL-8 and TGF-β in PJ-treated cells related to DMSO-treated cells and indicated by qRT-PCR. GAPDH-mRNA was used as an internal control for the qRT-PCR test. Error bars reveal the SD of three independent experiments. Student two-tailed t-test was used for significance analysis of Ct values indicated by qRT-PCR. (D) Immunoblotting assay indicates mutant K-Ras, B-Raf, and phospho-P53 proteins in PJ-treated cells compared to DMSO-treated cells. The calreticulin was served as an internal control. ^*P < 0.05 was considered statistically significant and ^**P < 0.01 as highly significant.