Fig. 3.

miR-133a-3p directly targets MYH9 to inactivate the Wnt/β-catenin signal. a Bioinformatics analysis revealed that the 3’UTR of MYH9 was well matched with the seed sequence of miR-133a-3p. Mutants were generated in the binding region of the MYH9 3’UTR. b MYH9 protein levels in miR-133a-3p-overexpressing/suppressing 5–8F cells and HONE1-EBV + cells were detected by western blot analysis. c MYH9 mRNA levels in miR-133a-3p-overexpressing/suppressing 5–8F cells and HONE1-EBV + cells were detected by qPCR assays. d miR-133a-3p directly targets MYH9, as confirmed by a dual-luciferase reporter assay. One-way ANOVA and Dunnett multiple comparison tests were used for analysis. *P < 0.05. Sphere-forming assay (e, scale bar: 500 μm), migration assay, invasion assays (f, scale bar: 200 μm) and IC50 (g) of NPC cells were performed after transfection with a FOXO1 lentiviral vector or miR-133a-3p inhibitor as indicated. Student’s t-tests were used for analysis. Mean ± s.d., *P < 0.05; **P < 0.01; ***P < 0.001. h Expression of MYH9, β-catenin, ZEB1, TCF4, N-Ca, E-Ca, and Vimentin following miR-133a-3p suppression in FOXO1-overexpressing NPC cells. Abbreviations: Mi-133a-3p: miR-133a-3p mimics; In-133a-3p: miR-133a-3p inhibitor