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. 2019 Nov 18;10(12):872. doi: 10.1038/s41419-019-2105-0

Fig. 2. FGF21 upregulates angiogenesis in flaps.

Fig. 2

On the 7th day after operation, the samples were harvested for the evaluation of angiogenesis in ischemic skin flaps. a, c IHC of proteins VEGF and Cadherin5 in ischemic skin flaps (original magnification, ×200; scan bar, 50 μm). b, d Quantification of integral absorbance of VEGF and Cadherin5 in IHC. e, f, g Western blotting performed for expression of MMP9, VEGF, and Cadherin5, which was corrected by GAPDH as internal control. h, i, j Quantization of optical density values of MMP9, VEGF and Cadherin5 expressions in the flaps. Significance: *p < 0.05 and **p < 0.01 vs. Control group. Data were expressed as means ± SEM (n = 6 per group).