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. 2019 Nov 18;10(12):872. doi: 10.1038/s41419-019-2105-0

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 5 — On the 7th day after operation, the samples were harvested for the evaluation of autophagy. a Immunofluorescence for the assessment of LC3II in the ischemic skin flap (scan bar, 15 μm). b Quantification of percentage of positive cells with LC3II labeled autophagosomes in dermal layer. (c) CTSD expression in ischemic skin flap evaluated by IHC. d Histogram showing expression of CTSD detected by IHC. e, f Western blotting for expression of Belin1, LC3II, CTSD, VPS34. and p62, which was corrected by GAPDH as internal control. g Histogram showing the levels of Belin1, LC3II, CTSD, VPS34, and p62 detected by western blotting. Significance: *p < 0.05 and **p < 0.01 vs. the Control group. Data were expressed as means ± SEM (n = 6 per group).