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. 2019 Nov 18;10(12):873. doi: 10.1038/s41419-019-2114-z

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a Representative images of hematoxylin–eosin staining and HIC-5 staining in esophageal squamous cell cancer tissue and adjacent non-tumor tissue (scale bar, 500 μm and 200 μm (right panels)). The red arrows represent tumor stroma. b Cellular co-localization of αSMA and HIC-5 in ESCC tissue and adjacent non-tumor tissue according to immunofluorescence analysis (scale bar, 200 μm). c Identification of isolated CAFs and paired NFs from tumor tissue and adjacent non-tumor tissue according to immunofluorescence staining for αSMA, FN1, and vimentin (scale bar, 50 μm). d Relative mRNA expression of HIC-5 in CAFs, NFs, KYSE150, and TE1 cells by qRT-PCR. e Western blot analysis of HIC-5, αSMA, and FN1 in NFs and CAFs. f Comparison of HIC-5 H-SCORE in 80 pairs of human ESCC tissue and adjacent non-tumor tissue. g Representative images of HIC-5 staining in esophageal squamous cell metastatic cancer and nonmetastatic cancer (scale bar, 50 μm). The red asterisks represent tumor stroma. The data represent the mean ± SEM of three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.