Figure 3.
Conditioned medium from turtle gut bacteria demonstrated significant antibacterial effects against selected Gram-negative bacteria. Briefly, 1 × 106 bacteria were incubated with turtle gut bacterial CM for 2 h at 37°C. Following this, serial dilution and plating onto nutrient agar plates were performed and plates were incubated overnight at 37°C. Bacterial colonies were enumerated on the following day. The data are expressed as the mean ± standard error of several independent experiments performed in duplicate. T-test statistics was performed to find P values, (*) is P ≤ 0.05. Bacteria incubated with gentamicin (100 µg/mL) while with PBS and CM5 were taken as positive and negative controls. (a) CM tested against E. coli K1, (b) against P. aeruginosa (c) against K. pneumoniae (d) against S. enterica, and (e) against S. marcescens. CM1 is E. cloacae, CM2 is A. hydrophila, CM3 is P. aeruginosa, CM4 is P. aeruginosa and CM5 is E. coli K-12.