Table 4.
Validation of genes from microarray in control and haze predisposed cohort using real time quantitative PCR.
| Functional pathways | Selected genes | Patient cohort gene expression (qPCR values) | ||
|---|---|---|---|---|
| Controls | Haze predisposed | p Values Control vs Haze predisposed | ||
| Signaling factor | FOS | 0.5 ± 0.13 | 0.88 ± 0.1 | 0.23 |
| Extra cellular matrix related | NID1 | 1.13 ± 0.25 | 2.73 ± 0.39 | 0.01 |
| LAMA1 | 1.28 ± 0.43 | 3.62 ± 0.75 | 0.02 | |
| LAMA5 | 32.81 ± 7.14 | 23.18 ± 6.8 | 0.45 | |
| COL3A1 | 6.55 ± 2.82 | 0.6 ± 0.2 | 0.09 | |
| COL5A1 | 4.36 ± 1.3 | 2.92 ± 0.26 | 1.00 | |
| COL5A2 | 20.91 ± 3.51 | 13.55 ± 2.89 | 0.19 | |
| COL5A3 | 48.2 ± 10.28 | 44.18 ± 4.48 | 0.83 | |
| COL6A1 | 3.31 ± 0.92 | 1.5 ± 0.84 | 0.19 | |
| COL12 | 81.96 ± 15.34 | 40.27 ± 9.1 | 0.04 | |
Table consists of gene names and the regulated fold change observed in the patient cohort consisting of control (normal PRK who does not develop haze) and haze predisposed (normal PRK who develop haze). Highlighted in bold p-value represents statistical significance.