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. 2019 Nov 18;9:16970. doi: 10.1038/s41598-019-53174-6

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© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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miR-4715-3p overexpression increased sensitivity to cisplatin. ATP-glo cell viability assay analysis of OE33 (A) and MKN45 (B) cells, following transient transfection with miR-4715-3p. After 48 h, cells were treated with or without cisplatin 5 µM overnight. Flow cytometry analysis of Annexin V positive cells in OE33 and MKN45 cells with miR-4715-3p overexpression or negative control miRNA. (C,D) left panels show representative flow cytometry profiles and right panels display bar graph of live and apoptotic cells. *p < 0.05, **p < 0.01, ****p < 0.0001.