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. 2019 Nov 12;10:1111. doi: 10.3389/fgene.2019.01111

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Copyright © 2019 Guo, Meng, Zhai, Xie, Zhao, Li, Zhou, Li, Liu, Yang and Wu

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Constructs generated to validate the protein-coding potential of small open reading frames. The 5′UTR-ORFs of glyceraldehyde 3-phosphate dehydrogenase, ZFAS1, SNHG8, and RP11-879F14.2 were fused to a GFPmut in which the green fluorescent protein start codon ATGGTG was mutated to ATTGTT. Substantial amounts of fusion protein were detected following transfection of the constructs into SK-Hep1 cells. In addition, the fusion protein was abolished after SK-Hep1 cells were transfected with the GFPmut construct.