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. 2019 Nov 12;10:768. doi: 10.3389/fendo.2019.00768

Figure 5.

Figure 5

Anti-inflammatory effects of CNTF in hMADS adipocytes. (A) MCP1, IL-6, IL-1β, and PAI1 mRNA levels in hMADS adipocytes treated for 24 h with 10 ng/ml TNFα alone or administered with 1 nM CNTF for 24 or 48 h. Data (n = 3) are mean ± SEM, *p < 0.05 compared with untreated cells (NT), #p < 0.05 compared with cells treated with TNFα. Data were analyzed using one-way ANOVA. 2-NBDG uptake, expressed as mean fluorescent intensity (MFI), (B) and representative immunoblot and quantification of 473-serine AKT phosphorylation (C) in hMADS adipocytes treated with 10 ng/ml TNFα for 24 h; with 100 nM insulin for 20 min; with TNFα and insulin; or with TNFα, insulin and 1 nM CNTF for 24 or 48 h. Data (n = 3) are mean ± SEM, *p < 0.05 compared with untreated cells (NT), $p < 0.05 compared with cells treated with insulin, #p < 0.05 compared with cells treated with insulin and TNFα. Data were analyzed using one-way ANOVA.