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. 2019 Nov 12;10:1329. doi: 10.3389/fphar.2019.01329

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Copyright © 2019 Fan, Tang, Ye, Zhu, Dai, Yao and Yao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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QLQX inhibits apoptosis and excessive autophagy induced by ISO in H9c2 cells. (A) Representative images of the total apoptotic cells by annexin V-FITC/PI detection. (B) Quantitative analysis of apoptotic cells using bar graphs. (C) Cellular autophagy measured by Cyto-ID autophagy detection reagent by flow cytometry. (D) Quantitative analysis of mean fluorescence intensity in different groups. (E) Representative western blotting bands of Bcl-2 and Bax. (F) Quantitative analysis of the ratio of Bcl-2/Bax by densitometry based on immunoblot images. (G) Representative western blotting bands of LC3I, LC3II and P62. (H, I) Quantitative analysis of the ratios of LC3II/LC3I and P62/GAPDH by densitometry based on immunoblot images. Data are expressed as mean ± SD, n = 3. ^### p 0.001 versus control group; **p < 0.01, ***p < 0.001 versus ISO group. ISO, isoproterenol; QLQX, Qi-Li-Qiang-Xin.