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. 2019 Nov 12;10:1329. doi: 10.3389/fphar.2019.01329

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Copyright © 2019 Fan, Tang, Ye, Zhu, Dai, Yao and Yao

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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The role of AKT/mTOR pathway in cardioprotective effect of QLQX in vitro. (B) Representative western blotting bands of p-AKT, AKT, p-mTOR and mTOR. (B, C) Quantitative analysis of the ratios of p-AKT/AKT, p-mTOR/mTOR by densitometry based on immunoblot images. (D) Representative images of the total apoptotic cells by annexin V-FITC/PI detection. (E) Quantitative analysis of apoptotic cells using bar graphs. (F) Representative western blotting bands of LC3I, LC3II, p-AKT, AKT, p-mTOR and mTOR. (G, H, I) Quantitative analysis of the ratios of LC3II/LC3I, p-AKT/AKT, p-mTOR/mTOR by densitometry. Data are expressed as mean ± SD, n = 3. ^# p < 0.05, ^## p < 0.01^,### p 0.001 versus control group; *p < 0.05, **p < 0.01, ***p < 0.001 versus ISO group. ISO, isoproterenol, QLQX, Qi-Li-Qiang-Xin.