Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2019 Nov 18;9:16958. doi: 10.1038/s41598-019-53263-6

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2019

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Glia cell activation and myelin damage were assessed from day 1 to day 7 after ICH. (A) The schematic diagrams show the four areas (white squares) for immunofluorescence photography in the perihaematomal region, and the white star indicates the haematoma. Iba1, GFAP-positive cells and MBP in brain sections were identified on days 1, 3 and 7 after ICH. Immunoreactivity of Iba1 (ionized calcium-binding adaptor molecule 1) and GFAP (glial fibrillary acidic protein; astrocyte marker) is shown in green, MBP (myelin basic protein) is shown in red, and NF-200 (neurofilament-200) is shown in green. Nuclei were stained with DAPI (blue). Scale bars: 50 μm (inset column). (B) Intensity of Iba-1, GFAP and MBP staining and diameter of NF-200 were quantified; n = 3 mice and 6 pictures per time point. (C) TEM shows the myelin sheath around the haematoma on day 3 after ICH. (D) G-ratio of different groups (n = 60, 3 mice). (E) Diameter of axons (n = 6 slices, 3 mice). Values are shown as the mean ± S.D., *P < 0.05, **P < 0.01, ***P < 0.001; ^†P < 0.05 vs. sham.