. 2019 Nov 18;9:16974. doi: 10.1038/s41598-019-53619-y

Table 1.

Summary statistics for results of real-time PCR assay using SSL-3 and 18S ribosomal gene primers with DNA templates of Botryococcus braunii strains.

Clade^†	N	Ct^SSL3	Ct^18S	ΔCt	2^−Δ(ΔCt)
A	3	31.4 (0.85) 31.6	32.8 29.9	19.1 (1.3) 19.9	20.8 16.5	12.4 (1.8) 12.8	15.2 9.1	0.0075 (0.0065) 0.0015	0.021 0.00050
L	11	33.3 (0.42) 33.4	35.1 30.4	19.7 (0.12) 19.6	20.2 19.3	13.6 (0.40) 14.0	15.5 11.1	0.0020 (0.0005) 0.0011	0.0056 0.00040
S	7	33.3 (0.73) 33.9	35.7 30.0	19.5 (0.53) 19.5	22.4 18.2	13.8 (0.89) 13.6	17.6 10.6	0.0021 (0.0009) 0.0015	0.0073 0.000095
B₁	12	25.0 (0.28) 25.0	26.5 23.4	20.1 (0.26) 20.2	21.6 18.9	4.9 (0.15) 5.0	5.4 3.6	0.60 (0.05) 0.57	0.92 0.36
B₂	37	25.1 (0.24) 24.8	30.3 23.2	20.8 (0.25) 20.3	26.5 19.1	4.4 (0.06) 4.4	4.9 3.0	0.89 (0.03) 0.86	1.38 0.58
Showa^§		23.5 (0.25)		19.3 (0.27)		4.2 (0.012)		1

Clade^†

Ct^SSL3

Ct^18S

ΔCt

2^−Δ(ΔCt)

Average (SE)
Median

Max
Min

Average (SE)
Median

Max
Min

Average (SE)
Median

Max
Min

Average (SE)
Median

Max
Min

31.4 (0.85)

31.6

32.8

29.9

19.1 (1.3)

19.9

20.8

16.5

12.4 (1.8)

12.8

15.2

9.1

0.0075 (0.0065)

0.0015

0.021

0.00050

33.3 (0.42)

33.4

35.1

30.4

19.7 (0.12)

19.6

20.2

19.3

13.6 (0.40)

14.0

15.5

11.1

0.0020 (0.0005)

0.0011

0.0056

0.00040

33.3 (0.73)

33.9

35.7

30.0

19.5 (0.53)

19.5

22.4

18.2

13.8 (0.89)

13.6

17.6

10.6

0.0021 (0.0009)

0.0015

0.0073

0.000095

B₁

25.0 (0.28)

25.0

26.5

23.4

20.1 (0.26)

20.2

21.6

18.9

4.9 (0.15)

5.0

5.4

3.6

0.60 (0.05)

0.57

0.92

0.36

B₂

25.1 (0.24)

24.8

30.3

23.2

20.8 (0.25)

20.3

26.5

19.1

4.4 (0.06)

4.4

4.9

3.0

0.89 (0.03)

0.86

1.38

0.58

Showa^§

23.5 (0.25)

19.3 (0.27)

4.2 (0.012)

^†Clade is classified based on the molecular phylogeny of 18S ribosomal sequence.

^§Average (SE) of Ct values with Showa DNA template (N = 3).

ΔCt = Ct^SSL3 – Ct^18S.

Δ(ΔCt) = ΔCt –ΔCt^Showa.