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. 2019 Nov 12;11:308. doi: 10.3389/fnagi.2019.00308

Figure 1.

Figure 1

Tau protein expression and tau phosphorylation in the hippocampus post-status epilepticus. (A) Illustration of intra-amygdala injection of Kainic acid (KA) in mice for the induction of status epilepticus and chronic epilepsy. Flouro-jade B (FjB) staining shows neurodegeneration in the ipsilateral hippocampus at 24 h post-KA injection. Note: absence of FjB-positive cells at 4 h post status epilepticus (n = 4 per group). FjB-positive cells are mainly localized to the ipsilateral CA3 subfield of the hippocampus. Scale bar = 200 μm. (B) Tau mRNA levels quantified by RT-qPCR showing no changes between control and post-status epilepticus (n = 4 per group). (C–E) Representative Western blot (n = 1/lane) and corresponding graphs showing an increase in total tau (Tau-1; Ctrl vs. 4 h, p = 0.03), AT8/Tau-1 (Ctrl vs. 4 h, p = 0.03; Ctrl vs. 8 h, p = 0.005) and PHF-1/Tau-1 protein levels in the ipsilateral hippocampus post-status epilepticus. GAPDH is shown as a loading control (n = 4 per group). One-way ANOVA with Fisher’s post hoc test. Data are presented as mean ± standard error of the mean (SEM). *p < 0.05, **p < 0.01, ***p < 0.001.