Table 1.
Nucleotide sequences of preamplification primers.
| Target gene | Primers | Sequences | Tm (°C) |
|---|---|---|---|
| Chlamydia trachomatis | SC-STD1-F | gggttccctaagggttggaGGGAATCCTGCTGAACCAA | 61.0 |
| SC-STD1-R | gtgccagcaagatccaatctagaTCAAAACACGGTCGAAAACA | 60.0 | |
| Neisseria gonorrheae | SC-STD3-F | gggttccctaagggttggaCGGCAGCATTCAATTTGTT | 59.0 |
| SC-STD3-R | gtgccagcaagatccaatctagaAAAAAGCCGCCATTTTTGTA | 58.5 | |
| Trichomonas vaginalis | SC-STD5-F | gggttccctaagggttggaCCAGAAGTGGGCTACACACC | 63.0 |
| SC-STD5-R | gtgccagcaagatccaatctagaATACCAAGGCCGGAAGCAC | 64.0 | |
| Herpes simplex virus II | SC-STD7-F | gggttccctaagggttggaCATGGGGCGTTTGACCTC | 62.4 |
| SC-STD7-R | gtgccagcaagatccaatctagaTACACAGTGATCGGGATGCT | 62.2 | |
| Mycoplasma genitalium | SC-STD4-F | gggttccctaagggttggaACCTTGATGGTCAGCAAAACTT | 62.0 |
| SC-STD4-R | gtgccagcaagatccaatctagaCCTTTGATCTCATTCCAATCAGTA | 59.5 | |
| Herpes simplex virus I | SC-STD6-F | gggttccctaagggttggaCTGTGGTGTTTTTGGCATCA | 60.7 |
| SC-STD6-R | gtgccagcaagatccaatctagaGGTTGTGGAGGAGACGTTG | 61.8 | |
| Ureaplasma urealyticum /Ureaplasma parvum | SC-STD9/10-F | gggttccctaagggttggaGCTGACGTTGCAAGAAGACG | 63.6 |
| SC-STD9/10-R | gtgccagcaagatccaatctagaACCATCAGGGAAAGTAACTTCAAC | 62.0 | |
| Internal control | SC-IAC-F | gggttccctaagggttggaACATGTAACCGCCCCCATT | 63.2 |
| SC-IAC-R | gtgccagcaagatccaatctagaTCCACGCACGCACTACTATG | 63.7 | |
| Mycoplasma hominis | SC-MH–F | gggttccctaagggttggaGGAAGATATGTAACAAAAGAAGGTGCTG | 63.3 |
| SC-MH-R | gtgccagcaagatccaatctagaTTTATCTTCTGGCGTAATGATATCTTCG | 62.2 | |
| General primer | Primer-F | gggttccctaagggttgga | 62.2 |
| Primer-R | gtgccagcaagatccaatctaga | 63.6 |
Lowercase letters indicate general primer, uppercase letters indicate specific primer. Tm value is calculated using only the specific primer portion, excluding the general primer portion.