Table 2.
Nucleotide sequences of pathogen-specific regions of the probes.
| Probes | Target gene | Sequences | Channels | Tm (°C) |
|---|---|---|---|---|
| SC-MH-HEX | Mycoplasma hominis | ACTGCTCCAGCTAAAAGCGAA | 557 ± 5 | 62.5 |
| SC-STD5-HEX | Trichomonas vaginalis | TCTGGCAAGATCAAGGACATCCTCC | 557 ± 5 | 67.0 |
| SC-STD9/10-ROX | Ureaplasma urealyticum/ Ureaplasma parvum | CTTTAATTACTGATCATGTAATGGAAGGGGCA | 610 ± 5 | 56.5/66.0 |
| SC-STD7-ROX | herpes simplex virus II | TGAAGCGTGTTTACCACATTCAGCCGAGCCTG | 610 ± 5 | 74.0 |
| SC-STD3-FAM | Neisseria gonorrheae | AGTAATCAGATGAAACCAG | 510 ± 5 | 54.5 |
| SC-STD4-FAM | Mycoplasma genitalium | AGCCTATCTTTGATCCTTTTAAAGGCTTTGG | 510 ± 5 | 66.0 |
| SC-STD6-Cy5 | herpes simplex virus I | TTATCCCATTCCTTTTGGTTCTTGTCGGTG | 660 ± 10 | 68.5 |
| SC-STD1-Cy5 | Chlamydia trachomatis | CGCTATCAGCATGCGT | 660 ± 10 | 57.5 |
| SC-IAC-HEX | Internal control | GAGAGGCCGCACCTTGGTAGTAAATAGACACATGGCCGAGT | 557 ± 5 | 76.0 |
Tm value is calculated using the full length of the probe.