Table 4.
Performance of novel multiplex real-time PCR assay compared to Sanger sequencing in Hospital B.
| Pathogene | Sensitivity (95% CI) | Specificity (95% CI) | Kappa value | Kappa P | McNemer P |
|---|---|---|---|---|---|
| Chlamydia trachomatis | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| Neisseria gonorrhoeae | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| Trichomonas vaginalis | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| HSVI | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| HSVII | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| Mycoplasma genitalium | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| Ureaplasma urealyticum | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
| Ureaplasma parvum | 99.32% (98.42–100.21%) | 98.35% (96.97–99.73%) | 0.975 | <0.001 | 0.625 |
| Mycoplasma hominis | 100% (100–100%) | 100% (100–100%) | 1.000 | <0.001 | 1.000 |
Trichomonas vaginalis.