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. 2019 Nov 12;9:383. doi: 10.3389/fcimb.2019.00383

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Copyright © 2019 Leroy, Bortolus, Lecointe, Parny, Charlet, Sendid and Jawhara.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Chitin reduced platelet aggregation and intracellular calcium concentration. (A) Representative aggregation curve. Untreated platelets or platelets pre-treated with chitin at different concentrations (50, 100, or 200 μg/mL) were activated with 0.2 U/mL thrombin. (B) Percent maximal aggregation in the presence of thrombin. Data are the mean ± SD of three independent experiments (n = 3–4 per group). (C) Fura-2 fluorescence reflecting cytosolic Ca²⁺ concentration [Ca²⁺] of platelets pre-treated with chitin and stimulated with thrombin.