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Chitin increases TLR8 expression in thrombin-stimulated platelets. (A–D) Relative expression levels of TLR2, TLR4, TLR8, and TLR9 mRNA, respectively, in platelets by real-time PCR. CTL– (control) corresponds to unstimulated platelets, CTL+ (control) represents platelets stimulated with thrombin (0.05 U/mL). 50 μg/mL corresponds to chitin concentration. Data are the mean ± SD of three independent experiments.
