Table 1.

Effect of phagocytosis and phagosomal acidification inhibition on DC cytokine expression induced by live and inactivated USA300 preparations.

	Treatment	pg/ml	+Cytochalasin D (5 μM)	+ Chloroquine (2 μM)
IL-12	USA300 live 0.1	4,668 ± 335	6.3 ± 61**	838 ± 5901**
	USA300 HI 1	1,038 ± 30	0.3 ± 0.31***	239 ± 51***
	USA300 UV 1	5,342 ± 931	Not det	3,960 ± 2,294
	USA300 PFA 1	6,704 ±2,768	3 ± 3	2,414 ± 2,052
TNF-α	USA300 live 0.1	13,013 ± 1,881	83 ± 451**	2,138 ± 7211**
	USA300 HI 1	872 ± 507	16 ± 3	411 ± 208
	USA300 UV 1	6,473 ± 2,432	21 ± 7	3,798 ± 55
	USA300 PFA 1	6,074 ± 1,196	27 ± 141*	2,279 ± 8451**
IL-6	USA300 live 0.1	11,465 ± 2,518	55 ± 111**	2,088 ± 1,217
	USA300 HI 1	1,793 ± 245	26 ± 91**	572 ± 1201**
	SA300 UV 1	6,008 ± 653	53 ± 191**	3,647 ± 628
	USA300 PFA 1	6,235 ±1,716	53 ± 121**	3,309 ± 1,3701**
IL-23	USA300 live 0.1	1,008 ± 227	2 ± 11*	167 ± 411*
	USA300 HI 1	69 ± 39	26 ± 18	13 ± 7
	USA300 UV 1	481 ± 185	Not det	24 ± 24
	USA300 PFA 1	393 ± 321	20 ± 20	22 ± 15
IL-10	USA300 live 0.1	269 ± 139	3 ± 1.4	59 ± 24
	USA300 HI 1	6 ± 2	1.3 ± 1 3	3 ± 1.5
	USA300 UV 1	64 ± 29	1 ± 1	27 ± 15
	USA300 PFA 1	47 ±22	1 ± 0.7	17 ± 10
IL-8	USA300 live 0.1	18,575 ± 434	5,849 ± 1,6161**	14,994 ± 5,299
	USA300 HI 1	5,398 ± 1,270	6,162 ± 2,934	3,439 ± 749
	USA300 UV 1	6,366 ± 546	3,100 ± 972	3,826 ± 384
	USA300 PFA 1	5,308 ± 819	6,848 ± 3,666	4,057 ± 1,323
IL-1β	USA300 live 0.1	129 ± 41	4 ± 2	37 ± 8
	USA300 HI 1	4 ± 4	Not det	1 ± 1
	USA300 UV 1	14 ± 7	Not det	8 ± 4
	USA300 PFA 1	14 ± 6	2 ± 2	4 ± 2

DC were pre-treated with 5 μM Cytochalasin D or with 2 μM Chloroquine for 30 min and then stimulated for 24 h with live and differentially inactivated USA300 strain. The secretion of the indicated cytokines were measured in DC culture supernatants by Inflammatory Cytokine array kit. The results represent means ± SEM of three independent experiments (

^*p < 0.05;

^**p < 001;

^***p < 0.001).