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. 2019 Nov 12;7:267. doi: 10.3389/fcell.2019.00267

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Copyright © 2019 Wu, Tang, Yin, Yan, Tang, Xin, Pan, Ma and Yan.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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AT-MSC augments the effects of RT against HCC cells proliferation and sphere-forming. (A) Cell viability of Huh7 and HepG2 cells treated with radiation and/or AT-CM for 12, 24, 48, and 60 h was assessed by CCK8 assay. Data represent the means ± SD of three independent experiments (^∗∗P < 0.01, ^∗∗∗P < 0.001). (B,C) Huh7 cells treated with RT with or without AT-CM were cultured on ultra-low attachment plates to assess sphere-formation ability. Representative images are shown. The results are expressed as means ± SD of three independent experiments (^∗P < 0.05, ^∗∗P < 0.01, ^∗∗∗P < 0.001). (D) The effect of AT-MSCs co-culture with human HCC cell line Huh7 and HepG2 after irradiation. Tumor cells grown as colonies in a monolayer culture. The initial size of the colonies was measured and successive treatments was applies for 7 days more.