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. 2019 Nov 12;7:267. doi: 10.3389/fcell.2019.00267

FIGURE 3.

FIGURE 3

AT-CM in combination with RT against HCC cells colony-forming, wound-healing, migration, and invasion. (A,B) The colony-formation assay showed decreased tumorigenicity in Huh7 cells, which treated with RT and AT-CM combination therapy. The graph shows the mean integrated density of colonies normalized by 100%. (C) Tumor cells exposed to irradiation with or without AT-CM were fixed and then CD44, CD90, and CD133-positive cells were analyzed with FACS. Data are from representative experiments repeated at least three times. (D) Representative wound healing assays (12 and 24 h) after scratching (0 h) demonstrated that the combination treatment reduced the wound closure ability of Huh7 cells. Representative images are shown (magnification 100 ×). (E–H) Transwell migration and invasion assays were performed to compare the motility of HCC cells treated with RT with or without AT-CM. The numbers of migrated (top) and invading (bottom) cells were calculated and are depicted in the bar chart (∗P < 0.05, ∗∗P < 0.01, ∗∗∗P < 0.001).