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. 2019 Nov 12;7:267. doi: 10.3389/fcell.2019.00267

FIGURE 7.

FIGURE 7

IFITM1 interacts with STAT3 and MMPs and inhibits downstream p53 and Caspases. (A) Control (IF-NC) and IFITM1-overexpression (IF-OE) Huh7 cells treated with RT with or without AT-CM were cultured on ultra-low attachment plates to assess sphere-formation ability. Representative images are shown. (B) The results are expressed as means ± SD of three independent experiments (∗P < 0.05, ∗∗P < 0.01). (C) The colony-formation assay showed increased tumorigenicity in IF-OE Huh7 cells. (D) The graph shows the mean integrated density of colonies normalized by 100% (∗∗P < 0.01, ∗∗∗P < 0.001). (E) We overexpressed IFITM1 in Huh7 cells using lentivirus-mediated transcription, and the expression level of IFITM1 was assessed by Western blot analysis using β-Actin as loading control. (F) IF-NC and IF-OE Huh7 cells treated with RT with or without AT-CM and cell lysates were isolated to probe with antibodies indicated. Protein level was determined by western blot with β-actin used as the loading control. Representative images were shown. (G) Cell lysates were isolated from HCC Huh7 cells after knocking down IFITM1, and the protein levels were probed with antibodies indicated. β-actin used as the loading control. Representative images were shown.