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. 2019 Nov 20;44(4):233–241. doi: 10.1584/jpestics.D19-037

Fig. 2. Effect of EH-8 and its analogues on inducing the morphology typical of the triple response in Arabidopsis seedlings. The triple response of Arabidopsis seedlings was measured by determining the hypocotyl length, root length, and curvature of the hook. Approximately 50 seeds/well of Arabidopsis were grown for five days in the dark in a 24-well plate on medium containing 1/2 MS, and the concentration of the test compounds, including ethephon and the reference compound EH-8, was set at 10 µM. Ethephon was used as a positive control, while DMSO mock treatment was used as a control. (A) Effect of pyrazole derivatives on inducing exaggerated apical hooks of Arabidopsis seedlings; (B) effect of pyrazole derivatives on hypocotyl elongation; (C) effect of pyrazole derivatives on root growth. All the experiments in this work were conducted by measuring 11 seeds. Data are the means±S.E. obtained from 11 to 15 plants. All experiments were done three times to establish repeatability.

Fig. 2. Effect of EH-8 and its analogues on inducing the morphology typical of the triple response in Arabidopsis seedlings. The triple response of Arabidopsis seedlings was measured by determining the hypocotyl length, root length, and curvature of the hook. Approximately 50 seeds/well of Arabidopsis were grown for five days in the dark in a 24-well plate on medium containing 1/2 MS, and the concentration of the test compounds, including ethephon and the reference compound EH-8, was set at 10 µM. Ethephon was used as a positive control, while DMSO mock treatment was used as a control. (A) Effect of pyrazole derivatives on inducing exaggerated apical hooks of Arabidopsis seedlings; (B) effect of pyrazole derivatives on hypocotyl elongation; (C) effect of pyrazole derivatives on root growth. All the experiments in this work were conducted by measuring 11 seeds. Data are the means±S.E. obtained from 11 to 15 plants. All experiments were done three times to establish repeatability.