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. 2019 Nov 12;10:1189. doi: 10.3389/fneur.2019.01189

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Copyright © 2019 Wang, Zhang, Yu, Shen, Wu and Wu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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A diagram of the experimental design and brain blood flow after permanent middle cerebral artery occlusion (pMCAO). (A) A diagram of the experimental design. The mice underwent pMCAO on day 0. Enriched environment (EE) treatment lasted 3–21 days after pMCAO. The Morris water maze (MWM) test was performed 22–27 days after pMCAO. (B) Mouse brain was collected for Western blot analysis, immunohistochemistry, and transmission electron microscopy 21 days after pMCAO. (C) Representation of contralateral and ipsilateral blood flow after pMCAO (when the cerebral artery was occluded). *P < 0.05, n = 8 per group. (D) Pictures demonstrated EE (a) and standard housing (SH) (b).