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. 2019 Nov 12;10:2653. doi: 10.3389/fmicb.2019.02653

TABLE 1.

Analysis of variance (ANOVA) of Aspergillus flavus (Af) – Fusarium verticillioides (Fv) growth (mm) and aflatoxin B1 (AFB1) and fumonisin B1 + B2 (FBs) contamination (μg/kg) in the different treatments considered (fungi grown alone or together), temperature (10–40°C, step 5°C), time of incubation (3, 7, 10, 14, and 21 days) and medium (CMM or PDA).

Growth (mm) Mycotoxin (μg/kg)


Af Fv AFB1 FBs
Treatment ∗∗ n.s. n.s.
Alone 38.8 a 27.2 a 32,487 92,658
Together 35.3 b 11.9 b 25,191 57,981
Temperature (°C) ∗∗ ∗∗ ∗∗ ∗∗
10 0.0 f 0.1 e 6 d 0 d
15 26.7 d 35.2 b 28,175 b 30,929 ab
20 49.7 c 39.3 a 62,013 a 258,706 a
25 54.0 bc 37.4 ab 60,740 a 199,220 a
30 72.9 a 23.3 c 20,168 c 20,081 b
35 58.7 b 12.1 d 1,470 d 157 c
40 13.1 e 0.0 e 0 d 0 d
Time of incubation (days) ∗∗ ∗∗
3 23.4 c 12.4 c
7 29.2 c 16.8 b
10 40.4 b 15.3 b
14 43.0 ab 23.9 a
21 47.6 a 25.5 a
Medium n.s. ∗∗
CMM 41.6 14.4 b
PDA 32.6 24.7 a

n.s.: non-significant; p ≤ 0.05; ∗∗p ≤ 0.01.