TABLE 1.
Analysis of variance (ANOVA) of Aspergillus flavus (Af) – Fusarium verticillioides (Fv) growth (mm) and aflatoxin B1 (AFB1) and fumonisin B1 + B2 (FBs) contamination (μg/kg) in the different treatments considered (fungi grown alone or together), temperature (10–40°C, step 5°C), time of incubation (3, 7, 10, 14, and 21 days) and medium (CMM or PDA).
| Growth (mm) | Mycotoxin (μg/kg) | |||||||
| Af | Fv | AFB1 | FBs | |||||
| Treatment | ∗ | ∗∗ | n.s. | n.s. | ||||
| Alone | 38.8 | a | 27.2 | a | 32,487 | 92,658 | ||
| Together | 35.3 | b | 11.9 | b | 25,191 | 57,981 | ||
| Temperature (°C) | ∗∗ | ∗∗ | ∗∗ | ∗∗ | ||||
| 10 | 0.0 | f | 0.1 | e | 6 | d | 0 | d |
| 15 | 26.7 | d | 35.2 | b | 28,175 | b | 30,929 | ab |
| 20 | 49.7 | c | 39.3 | a | 62,013 | a | 258,706 | a |
| 25 | 54.0 | bc | 37.4 | ab | 60,740 | a | 199,220 | a |
| 30 | 72.9 | a | 23.3 | c | 20,168 | c | 20,081 | b |
| 35 | 58.7 | b | 12.1 | d | 1,470 | d | 157 | c |
| 40 | 13.1 | e | 0.0 | e | 0 | d | 0 | d |
| Time of incubation (days) | ∗∗ | ∗∗ | ||||||
| 3 | 23.4 | c | 12.4 | c | ||||
| 7 | 29.2 | c | 16.8 | b | ||||
| 10 | 40.4 | b | 15.3 | b | ||||
| 14 | 43.0 | ab | 23.9 | a | ||||
| 21 | 47.6 | a | 25.5 | a | ||||
| Medium | n.s. | ∗∗ | ||||||
| CMM | 41.6 | 14.4 | b | |||||
| PDA | 32.6 | 24.7 | a | |||||
n.s.: non-significant; ∗p ≤ 0.05; ∗∗p ≤ 0.01.