Table 1.
Lab-developed qRT-PCR primers and probe used for diagnostics of ZIKV.
| Name | Sequence (5′-3′) | Conc. (nM) | Target | PCR product (bp) | References |
|---|---|---|---|---|---|
| ZIKV_1086 | CCGCTGCCCAACACAAG | 600 | Envelope | 77 | (29) |
| ZIKV_1107 | FAM-AGCCTACCTTGACAAGCAGTCAGACACTCAA-BHQ1 | 100 | |||
| ZIKV_1162c | CCACTAACGTTCTTTTGCAGACAT | 600 | |||
| Zika2_fwd | CTTGGAGTGCTTGTGATT | 600 | NS2A | 187 | (34) |
| Zika2_ probe | FAM-AGAAGAGAATGACCACAAAGATCA-BHQ1 | 100 | |||
| Zika2_rev | CTCCTCCAGTGTTCATTT | 600 | |||
| PDV fwd | CGGGTGCCTTTTACAAGAAC | 600 | Heamagglutinine | 78 | (35) |
| PDV probe | Cy5-ATGCAAGGGCCAATT-MGB | 200 | |||
| PDV rev | TTCTTTCCTCAACCTCGTCC | 150 |
PDV, phocine distemper virus (internal control); BHQ, black hole quencher.