Table 3.
Primers And Annealing Temperatures For Antimicrobial Resistance Genes Amplification
| Gene | Primer Sequence | Annealing Temperature (°C) | Reference |
|---|---|---|---|
| blaCMY-2 | F: 5ʹ-ATAACCACCCAGTCACGC-3ʹ | 50 | 55 |
| R: 5ʹ-CAGTAGCGAGACTGCGCA-3ʹ | |||
| blaTEM-1 | F: 5ʹ-TTGGGTGCACGAGTGGGT-3ʹ | 56 | 56 |
| R: 5ʹ-TAATTGTTGCCGGGAAGC-3ʹ | |||
| tetA | F: 5ʹ-GCTACATCCTGCTTGCCTTC-3ʹ | 52 | 57 |
| R: 5ʹ-CATAGATCGCCGTGAAGAGG-3ʹ | |||
| tetC | F: 5ʹ-CTTGAGAGCCTTCAACCCAG-3ʹ | 42 | 57 |
| R: 5ʹ-ATGGTCGTCATCTACCTGCC-3ʹ | |||
| sul2 | F: 5ʹ-CGG CAT CGT CAA CAT AACC-3ʹ | 60 | 58 |
| R: 5ʹ-GTG TGC GGA TGA AGT CAG-3ʹ | |||
| dfrA7 | F: 5ʹ-AAATGGCGTAATCGGTAATG-3ʹ | 52 | 59 |
| R: 5ʹ-GTGAACAGTAGACAAATGAAT-3ʹ |