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. 2019 Nov 12;10:2498. doi: 10.3389/fmicb.2019.02498

Figure 4.

Figure 4

Fecal microbiota transplantation (FMT) treatment promotes CD4+ T cells but not CD8+ T cells in lamina propia lymphocytes (LPLs). (A–O) Single-cell suspensions of mouse LPL, mesenteric lymph nodes (MLN), and spleens from each group were prepared. Cells were stained with CD3-Percp-Cy5.5, CD4-FITC, CD8-APC-Cy7 for FACS analysis of CD3+CD4+ T cells (Th cells) or CD3+CD8+ T cells (Tc cells). Representative FACS gates from spleen (A), MLN (D), and LPL (G) are presented. The proportion and absolute numbers of Th cells (B,C,E,F,H,I) or Tc cells (J–O) in CD3+ T cells from splenocytes (B,C,J,K), MLN (E,F,L,M), and LPL (H,I,N,O) from each group were analyzed by FACS. Data are expressed as the mean ± SD of six mice for each group from one representative experiment. All experiments were repeated three times with similar results. ***P < 0.001 (ANOVA/LSD).