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Evaluation of CD49f Expression by Flow Cytometry
MDA-MB-231, CT26, and 4T1-iRFP720 cells were harvested, stained with BV421-labeled rat anti-human CD49f antibody or rat IgG2a κ isotype control, and gated cells were analyzed. (A–C) Representative histograms of MDA-MB-231 (A), CT26 (B), and 4T1-iRFP720 (C). (D) Geometric mean values for BV421 signal (n = 3 + SD).
