Figure 3.

Mean Cellular Association of Polyplexes

MDA-MB-231 cells were incubated with polyplexes based on Cy5-labeled pDNA prepared at N/P 9. Cells were treated in basal cell culture medium with two different concentrations and durations (1 μg/mL for 30 min and 4 h; 2 μg/mL for 30 min and 4 h), analyzed by flow cytometry, and the geometric mean values were calculated. Data are depicted as average value from three independent samples with 1 × 10⁴ per sample (n = 3 + SD; *p < 0 0.05; **p < 0.01; t-test, two-tailed). The background signal is shown as a dotted line.