Table 3.
An overview of some commercially available TS platforms.
| Platform | Company | Enrichment | Protocol overview |
|---|---|---|---|
| Ion AmpliSeq™ | Thermo Fisher Scientific | Amplicon | Targeted regions are amplified through target specific primers. These primers are removed, the sequencing adapters are added and the amplicons are amplified again to generate the library. Needs to be sequenced using Ion Torrent™ Sequencer. |
| Access Array | Fluidigm | Amplicon | Amplifies target regions, adding an overhanging universal adapter. The universal adapter is then bound by the sequencing adapters. Can be sequenced on both Ion Torrent™ and Illumina platforms. |
| HaloplexHS | Agilent | Amplicon | Circularises restriction enzyme fragmented gDNA using biotinylated probes. Probes captured using magnetic streptavidin beads. Circular molecules are then amplified to generate a linear library. |
| GeneRead DNAseq Targeted Panels V2 | Qiagen | Amplicon | Targeted regions amplified via multiplexed PCR-based enrichment. The samples are pooled, and the amplicons are purified using AMPure XP beads. Sequencing library can then be created using a platform specific kit. |
| TruSeq Amplicon | Illumina | Amplicon | Probes are bound at either end of a targeted region. The region is amplified via PCR, leaving an amplicon of the region with probes either end. Indices and sequencing adapters are then bound to the overhanging ends of the probes. |
| SureSelectXT | Agilent | Hybridization | Fragmented gDNA is amplified and the targeted regions are captured using target specific biotinylated probes. These probe-bound fragments are isolated and amplified to create the library. |
| SeqCap EZ | Roche Nimblegen | Hybridization | Fragmented gDNA is amplified, the sequencing adapters are added, and these fragments are then amplified. Target specific probes are added and probe bound fragments are isolated to generate the library. |
| Cell3™Target | Nonacus | Hybridization | DNA is enzymatically fragmented and Illumina Unique Molecular Identifier (UMI) containing adapters are ligated. The fragments are amplified prior to target enrichment using biotin-labelled probes and streptavidin coated beads. The enriched fragments are amplified again and then sequenced on an Illumina sequencer. |