Table 2.
Triclosan effects on mast cell degranulation pathway: Time to first signaling defect, following co-exposure to Ag+ TCS
| Signaling Event* | TCS Effect on this event** | Time of first defect caused by TCS*** | References |
|---|---|---|---|
| Efflux of Ca2+ from ER |  |
3 min | (Weatherly et al., 2018) |
| Influx of Ca2+ into mitochondria |  |
4 min | (Weatherly et al., 2018) |
| Mitochondrial Ca2+ oscillations |  |
10 min | (Weatherly et al., 2018) |
| Influx of Ca2+ into cytosol |  |
10 min (~10% )15 min (~20%  )1 hr (~50%  ) |
(Weatherly et al., 2018) |
| PKC βII and PKCδ translocation | Delay | Delayed from ~2.7 min (-TCS, + Ag) to ~4.2 min (+ TCS, +Ag) | Fig. 2C & 3C |
| PKC activity |  |
45 min | Fig. 1B |
| MARCKS translocation | Delay | Delayed from ~2.8 min (-TCS, + Ag) to ~4 min (+ TCS, +Ag) | Fig. 4C |
| Actin cytoskeleton ruffling |  |
10 min | Fig. 2D, 3D, 4D, & 6C |
| PLD1 translocation | Slight inhibition | 10 min | Fig. 6 |
| PLD activity |  |
15 min | Fig. 5A |
| Microtubule polymerization |  |
15 min | (Weatherly et al., 2018) |
| ROS formation |  |
~45 min | (Weatherly et al., 2018) |
*
Ag concentration used: 0.005 −0.1μg/mL. These doses caused roughly maximal degranulation in absence of TCS.
**
Items in red indicate mechanisms of TCS inhibition of degranulation.
***
TCS concentration used: 10–20μM