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. 2019 Oct 3;18:801–814. doi: 10.1016/j.omtn.2019.08.028

Figure 3.

Figure 3

Expression of miR-495 Is Reduced in LPS-Exposed NR8383 Cells due to the Methylation of miR-495 Promoter

(A) CpG island in the miR-495 gene promoter region predicted using the MethPrimer website. (B) Methylation of the miR-495 promoter in NR8383 cells treated with F-12K culture medium, LPS, combined LPS and DMSO, or combined LPS and 5-Aza as measured by MSP assay. (C) Enrichment of DNMT1 and DNMT3a/b in the miR-495 promoter region in NR8383 cells treated with F-12K culture medium, LPS, combined LPS and DMSO, or combined LPS and 5-Aza as measured by ChIP assay. (D) Expression of miR-495 in NR8383 cells treated with F-12K culture medium, LPS, combined LPS and DMSO, or combined LPS and 5-Aza as determined by qRT-PCR. (E) Methylation of miR-495 promoter in NR8383 cells treated with F-12K culture medium, LPS, combined LPS and DMSO, or combined LPS and 5-Aza detected by MeDIP assay. *p < 0.05 versus NR8383 cells treated with F-12K culture medium; #p < 0.05 versus NR8383 cells treated with LPS and DMSO. Measurement data were expressed as mean ± SD, and data among multiple groups were compared using one-way ANOVA and subjected to Tukey’s post hoc test. The experiment was repeated three times to obtain the mean value.