Table 1.
The Sequences of NLRP3-WT, NLRP3-Mut, and miR-495 Promoter for Dual-Luciferase Reporter Assay
| Gene names | Sequence |
|---|---|
| NLRP3-WT | GCGTGGAAGCAGGACCACCAGGTGCCTCGGTCCTGCCCCAAGTCCTGCCCCAAGCCCCAGTGCGCACTGCTCTTCACTGCTATCAAGCCCTCCTTCACCATCAGGATCACAGCCGAGGCTCTTCTGGTATAGGGTCTGGAGCAAAGGCTTGTGTGGGACCAAATATTTTTCCTCACATCGATAACGTGAAACTGCCAGAGGCTGCCCTTCCCATCATATCCTCAGTGGGCAAGGTGTTCCCTCTTGGTGACTTCATGGAAACAGCTTCAAGAAAACGCCTTTTCTGTCCTCCCCCGCCCTCCTCTTACTCCTGCCCCTCCTCCTCCTCCTCCTCCCCTCCCCCCCCCTCCTCCTCCGCTTCTCCCCCCACCTGTCTTTCTCTCTCTGGGCCTCTGGTTTTTTGACCTTTGCCCATACCTTCAGTCTTGTCTTCCTGTTAACTGACCATCCCGCATAAGGAGCTGCCCGTGGGCTAGATGGAAGGTTTGTGGCAGCCTCTCAGCTACATTGTTTGTTTTTATTTTTTAATAGTTATGATTTCTCTTTAGCTACCTGAAAACTCAGAGATTTATAAAACCCATTTTTGTATTTATTGTATGTTTGTACTGCTTTCTTAATTTAAAAATGTATCTAGAATTCTTTTAAGTTATTTATCCAAACTACTAAAAATAAATCAGTTTACACATTTAAAA |
| NLRP3-Mut | GCGTGGAAGCAGGACCACCAGGTGCCTCGGTCCTGCCCCAAGTCCTGCCCCAAGCCCCAGTGCGCACTGCTCTTCACTGCTATCAAGCCCTCCTTCACCATCAGGATCACAGCCGAGGCTCTTCTGGTATAGGGTCTGGAGCAAAGGCTTGTGTGGGACCAAATATTTTTCCTCACATCGATAACGTGAAACTGCCAGAGGCTGCCCTTCCCATCATATCCTCAGTGGGCAAGGTGTTCCCTCTTGGTGACTTCATGGAAACAGCTTCAAGAAAACGCCTTTTCTGTCCTCCCCCGCCCTCCTCTTACTCCTGCCCCTCCTCCTCCTCCTCCTCCCCTCCCCCCCCCTCCTCCTCCGCTTCTCCCCCCACCTGTCTTTCTCTCTCTGGGCCTCTGGTTTTTTGACCTTTGCCCATACCTTCAGTCTTGTCTTCCTGTTAACTGACCATCCCGCATAAGGAGCTGCCCGTGGGCTAGATGGAAGGTTTGTGGCAGCCTCTCAGCGCACGGCGGGCGGGTTATTTTTTAATAGTTATGATTTCTCTTTAGCTACCTGAAAACTCAGAGATTTATAAAACCCATTTTTGTATTTATTGTATGTTTGTACTGCTTTCTTAATTTAAAAATGTATCTAGAATTCTTTTAAGTTATTTATCCAAACTACTAAAAATAAATCAGTTTACACATTTAAAA |
| miR-495 Promoter | ACTCAGAATCCAGCCGCTTCGTGGGCAGCGTTCAGTCCATGCTTGGGGAAAGGGCTTTGT |
| TGCCGTTGCTCATCTCTGTCTGTTGTTCAGCTCTGCTTGCCTTACGAGGCCCCAGATGCC | |
| CTGGATGCCCTCAGTGTCAGACTGCGGAAGATGTGTCCCGGCTGTGACACGCGCTTCAGG | |
| GGAGGGCTGTTGGCGTCAGGTCCATGGAGAGGTATGGGAGTGGACATCTTGTCTGAGAGC | |
| AGACATTCTATTTCGGGTGTGTGTCTTGGTCCGTTCATCCTCAGTCTCCCTCCCATACCT | |
| AGGATGGCTTGTGGAGAGAAGGCCTGCCCTGAGGGCTGAAGTTGGGACCCACAGCTCTCA | |
| CCCCGGCCTTGGTATGCTCCCATGGCTACGGCAGAGGCACAAGAAAGTAATTAGAAACGC | |
| AGAAACTCCTTAAGGCCAAGGCTGGGAACTGCTATATTTCTACCCACAGATCATTGACCA | |
| GAACAATTACACGGCCGAACCCAAGGCAAGGGGCGGGGAGGCCCACTCCACCCGGGAGGG | |
| GGTTGTGGTGAGGTGTGAATGCAGGAACCGGTGAGGATTTGCCACCACCAAGTCAGTTGT | |
| CCACGTGGACCACCTGAGAACTTGGGTTTTATTCATTGCTCATTCATCCCTCCCTAACCC | |
| GCAGCTTCCGGTCTGCCCCGGGGGCTCTTCTCCGGCTCAGGCTGATCGACAGTGGTGACG | |
| CTGTGCCAGGACCCCTGGTCATTCCATCTGTTTGAGGCAAATGTTAAAAAATCCCTTGCG | |
| CTACCCATTCTATGACGACTCCCTTGGCTCAGAGGCTCCATCAGGGCAGACACTTTGCTG | |
| TTTGTCCCTAAAGGGTGGGTGGTAGATCTCATCCATCTTATCTGGGGAGTTATTGTGACT | |
| CTCCTGGTCCCTCCTCATGTATCCTCAGGAGGACTTTTAGGATGCAAAATCACTGTTTCT | |
| GTGCCATTGTCATATTCTGTGCTACTGGAGGAGACTTTTTGTTATTGAGAAATTGAACAC | |
| ATCGTGCTCACAAGCAGCTCCAGGACACAATGCCACCTGTGCAGAGGGGGCAGACCTGTT | |
| AGATGTCTCCCAGGTCTTGAGGGGGTTGTATGCACGGCCATATCTTTGTCACCGGTTGCC | |
| CGGTGCGCATCAGGACCCATGTGCTCTCAGGAAGCCCGCAGGAGTGATACCAGCCGCGGA | |
| GAAGCAGTGTTGTCAACGGTGCTGACGTGCGGTACTTAATGAGAAGTTGCCCGTGTTTTT | |
| TTCGCTTTATTTGTGACGAAACATTCGCGGTGCACTTCTTTTTCAGTATCCTATTCTGCC | |
| TTGAAGACGTCTTGGTTTGGGTGCAACTTCAGGGAAGGCACATGGGCCCCTGTAGTGGAC | |
| GCTGATCAGGACCTCCCTACCCCATGTCTTTTCTCCTTCCTAAATTTCTCCTTGTTTTTT | |
| TTTTTTTTTTTTTTTTTTATGAAAGCTCCCGTCTTCCCGCCGTGTGCTTTGATTGCATTG | |
| ACCCTGCCCCACTTCCTCCACCAGCCCAGGCTTAAGCGAATCTCTTGATCTCTTCTCCTT | |
| TGCCACTGCAATTGCTTCCGGAATAGGCCCATGACCCCACTGGGCCAGTGATAGGCAAGG | |
| AGATGCTTGCTGCAGCTTCTGGGAAAGAAACTGTTTCTCCCTTTCCTGTGATAGCTGCCA | |
| GAGTAGACACTGTCTATTCCATTCAGCTGGGAATAGACAGTGAACTTTGACGTGAGCTTC | |
| GAAGGTCCCACAGCCGTTTAAGGACTGAGAGGAGAGGGCTTGGTGCCACGCAGACACAGC | |
| AGAGGGAGTCTGTACCATGAAAGGCATCTTGGAGACCTAGAGAAGAGCCTGGATGATATC | |
| ATTTGAGTCCCTAGATCAAACCCTGCCTGAAGCTTGCTCTGTCTCTGTACTTTTTAGTTA | |
| TATACGTCTATAAATCCCTTACTTGTTTAAGCCAGTTGGAGTTAGATCTTATCTTACTTG | |
| CAATACAAACAGATGATACCACAGTGTGTTTTCCAGTGTGACTGGGAAAGCCTGCATTTT | |
| TTTCAATGATGTGTTTCTCACGGTTTGCCTATCTATCTTCAGTGTCCTATTTAAGGCAGG | |
| GTTTTGGTGGAAGGTGACTTTTCTTATGGACTTATGCTTTCAAAGCTTTGTCTTAGTGAT | |
| TTAATTTATGGTTGACAAATGATGCACAATTACCGTGTTTTGTGTCCAGCAATGGGTCAC | |
| TGGGTCCTCTCTGGAAGGCCTGATGGATGGTGGGCCCCAGAACTTCTGCCCCGATTCACT | |
| CTCCCTCGAATCCACCTCTCTCTGGGAGGCTGCCCTGCAAGTGATGTGGACTTGGGGAAG | |
| CCGTTGTCCTTTCCTGGCCCCTGCTGTCCTTTGGCTCTCTGTGGGTTGGCTGTATTTGTC | |
| ACAACTCACACTTAACTGTCAGTGTTTTAGAACCAAGCCATGCCTCCAGGGAACCCGGAG | |
| CACTTTAGAGAGGAGGTGCAGTGGTCAGCACCATGCAAGGTGCACAGAGAGATGGGTCCA | |
| CCGAGAGCCGGGGCTGCCTTGGTGGTTTGTTTCTCCTGATTCGATATCTTTGTCTTCCCA | |
| TCTCCTTCCTGGAACCATGGGCTCATGGAGGAGATTCTATTTTTGGGTCCAGGACCATCC | |
| CATTTTCTCTGAAGTGATGCTCTCCATGAGCATTTGCAGGGTTGATAGTAAACATTCCGA | |
| AAGCCGTCTGCCCTGATTATTGAATTCTGCCCATGAAGACTTCCTGGGATCCCGAAGTAG | |
| AGGGAGTCTGGGATCGGTTTGCTTTATCCGTGATGACTGTCCGCCTCTGCTCAGTGTCAG | |
| CCCAGCCCTTCGAAGCATGTCCCCCACCTCCCCTGGGGTGAGCCAACTTACCTGATGCTT | |
| TTAGGCTTAATTGAGCATAAGTTTGATTGGCAGCGTCTGCCCGGCCCTGCCCTCACGCCC | |
| TGACCCTCAGTGTCCCTTCACGCCCAGGTGTGCCTCTGGCAGGGAGGACGTGCTCATCTC |