Fig. 6.

Differences in oxidative stress between BH10 and vitamin K3. (A) Oxygen consumption rate (OCR) presented as a percentage of basal readings in MAD11 and HEC1A cells treated with vehicle (DMSO) or vitamin K3 (VitK3, 20 μM). Injection of vehicle and VitK3 indicated by arrow. (B) Representative western blots of peroxiredoxin 2 (PRDX2) and 3 (PRDX3) in MAD11 and HEC1A cells treated with vehicle (DMSO) and VitK3 (VK3, 20 μM) (150 min treatment). 14-3-3 serves as a protein loading control. (C) Oxidative DNA damage assay (measuring 8-hydroxy-2′-deoxyguanosine) performed on MAD11 and HEC1A cells treated with vehicle (DMSO), BH10 (20 μM) or VitK3 (20 μM) for 6 h. Data were analysed by 1-way ANOVA (C) with multiple comparison tests, where alpha = 0.05, *p ≤ 0.05, ***p ≤ 0.001, ns = not significant. Data in A and C represent mean ± SEM.