Fig. 3.

Heatmap of clustered DE genes identified in sea bass scales after treatment with E2 or Gen. The tree in the upper panel shows the hierarchical clustering of the DE genes (one gene/line) identified in the scales of the six treatment groups [injections with estradiol (E), genistein (G) or vehicle only (control, C) at 1 or 5 days (1d or 5d)]. The red gradient indicates high abundance, the green gradient indicates low abundance and black indicates equal abundance for each gene and condition relative to the average. 1 day after treatment the DE genes of the E2 group clustered more closely to the control than Gen1d. 5 days after treatment both E2-and Gen-treated scales clearly separated from the control and clustered together, suggesting a similar response at this time point.