Figure 1.

miR-214 Expression Correlates with Osteonecrosis of the Femoral Head

(A) Representative images of the femoral head. A, necrotic area; B, healthy (non-necrotic) area. (B) miR-214 levels were determined by RT-PCR and quantitative real-time PCR in necrotic and non-necrotic areas of human femoral heads with osteonecrosis. miR-214 levels were normalized to U6. Necrotic area group, n = 12; non-necrotic area group, n = 12. (C) Correlation analyses between mRNA levels of miR-214 and alkaline phosphatase (ALP), osteocalcin (BGLAP), collagen1 α1 (COL1α1) in bone specimens from patients with ONFH. miR-214 levels were normalized to U6. mRNA levels were normalized to GAPDH. For each group, n = 12. All data are presented as mean ± SEM. ∗∗p < 0.01. (D) Correlation analyses between mRNA levels of miR-214 and nuclear factor of activated T cells 1 (NFATc1), acid phosphatase 5 (ACP5), tartrate-resistant, cathepsin K (CTSK), matrix metallopeptidase 9 (MMP9), and chloride voltage-gated channel 7 (CLCN7) in bone specimens from patients with ONFH. miR-214 levels were normalized to U6. mRNA levels were normalized to GAPDH. Each group, n = 12. All data are presented as mean ± standard error of the mean (SEM) ∗∗P＜0.01.