Figure 4.

AAV-anti-RNA-214 Impedes Collapse of the Femoral Head in Osteonecrosis

(A) RT-PCR and quantitative real-time PCR analyses of miR-214 levels in the femoral head of rats among the groups at 2 and 8 weeks postsurgery. For each group, n = 6. (B) Representative three-dimensional images of the femoral head in the control, model, model + AAV-NC, and model + AAV-anti-miR-214 groups at 2 and 8 weeks postsurgery. Scale bar, 5 mm. For each group, n = 6. (C) Micro-computed tomography (CT) measurements of bone mineral density of the femoral heads from the control, model, model + AAV-NC, and model + AAV-anti-miR-214 groups at 2 and 8 weeks postsurgery. For each group, n = 6. (D) Micro-CT measurements of the ratio of trabecular bone volume to tissue volume of the femoral heads from the control, model, model + AAV-NC, and model + AAV-anti-miR-214 groups at 2 and 8 weeks postsurgery. For each group, n = 6. (E) H&E staining of rat femoral heads using paraffin sections from control, model, model + AAV-NC, and model + AAV-anti-miR-214 at 2 and 8 weeks postsurgery. Scale bar, 1 mm; for figures in black boxes, the scale bar represents 100 μm. All data are presented as mean ± SEM. *p < 0.05, **p < 0.01.