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. 2019 Nov 18;2(6):e201900464. doi: 10.26508/lsa.201900464

Figure 7. MHC-I recycles through a fast Arf6-independent pathway in BM-DCs.

Figure 7.

(A) C57BL/6 BM-DCs were pulsed with AF6 for 30 min on ice and shifted to 37°C for 30 min in the presence or absence of primaquine to allow for internalization and stained for AF6 using a secondary antibody. (A, B) BM-DCs were treated as in (A) including the internalization step for 30′, acid stripped, reincubated at 37°C in the absence of primaquine to allow for recycling, and finally stained with a secondary antibody for analysis by flow cytometry. (C) Transduced C57BL/6 BM-DCs were pulsed with AF6 as in (A) and then allowed to internalize AF6-bound H-2Kb for 60 min in the presence of primaquine. (B, D) Recycling of H-2Kb was analyzed as in (B), following an internalization step of 60 min. (A, B, C, D) Data represent means and SEM of n = 3 (A, B) and n = 4 (C, D) independent experiments. NT, nontargeting shRNA.