Figure S1. Detection of unfolded L^d molecules by microscopy and flow cytometry.

(A) shNT and shArf6-transduced Balb/c BM-DCs were stained for unfolded and folded L^d molecules at the cell surface. (B, C) shNT and shArf6-transduced Balb/c or C57BL/6 BM-DCs were stained for unfolded and folded L^d molecules intracellularly. (D, E) Balb/c BM-DCs were acid stripped and stained for folded and unfolded L^d molecules using mAbs 30 and 64, respectively, for analysis by immunofluorescence microscopy. (D, E, F) Control C57BL/6 BM-DCs were stained using mAbs 64 and 30 as in (D) and (E) and analyzed by confocal microscopy. Nuclei are stained with DAPI (blue). NT, non-targeting shRNA.