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. 2019 Oct;7(20):539. doi: 10.21037/atm.2019.10.07

Figure 2.

Figure 2

ES induces cell apoptosis in human lung carcinoma cells. (A) Apoptosis was analyzed by FACS using an Annexin V-FITC/PI cell apoptosis kit; (B) stained with Hoechst33342 solution and captured by fluorescence microscopy. White arrows indicate condensed nuclei; (C) expression levels of indicated proteins were detected by western blot assay; (D) pretreated with Z-VAD-FMK (50 µmol/L) for 1 h, apoptosis was analyzed by FACS using an Annexin V-FITC/PI cell apoptosis kit. All data are expressed as mean ± SD. *, P <0.05; **, P<0.01; ***, P<0.001 compared to the control group. ES, Ecliptasaponin A; FACS, fluorescence-activated cell sorting; PI, propidium iodide.